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Enzymes for synthesis of sequenced oligoamide triads and tetrads that can be polymerized into sequenced copolyamides.
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We tested 48 diverse homologs of SfaB and identified several enzyme variants that were more active than SfaB at synthesizing the nylon-6,6 monomer.
We have developed thermophilic bacterial strains that can break down PET and consume ethylene glycol and TPA. This will help enable modern, petroleum-derived plastics to be converted into value-added chemicals.
By engineering the Serine Integrase Assisted Genome Engineering (SAGE) genetic toolkit in an industrial strain of Aspergillus niger, we have established its proof of principle for applicability in Eukaryotes.
A pressure burst feature has been designed and demonstrated for relieving potentially hazardous excess pressure within irradiation capsules used in the ORNL High Flux Isotope Reactor (HFIR).
This manufacturing method uses multifunctional materials distributed volumetrically to generate a stiffness-based architecture, where continuous surfaces can be created from flat, rapidly produced geometries.
The lack of real-time insights into how materials evolve during laser powder bed fusion has limited the adoption by inhibiting part qualification. The developed approach provides key data needed to fabricate born qualified parts.
We present a comprehensive muti-technique approach for systematic investigation of enzymes generated by wastewater Comamonas species with hitherto unknown functionality to wards the depolymerization of plastics into bioaccessible products for bacterial metabolism.
Detection of gene expression in plants is critical for understanding the molecular basis of plant physiology and plant responses to drought, stress, climate change, microbes, insects and other factors.
This technology identifies enzymatic routes to synthesize amide oligomers with defined sequence to improve polymerization of existing materials or enable polymerization of new materials. Polymers are generally composed of one (e.g. Nylon 6) or two (e.g.