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Genetic modification of microbes that are thermophiles—ones that grow at elevated temperatures—is extremely challenging. Tools developed for E. coli, a typical host for protein production, typically do not function at elevated temperatures.

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Ionic liquids comprising quaternary ammonium and phosphonium cations have been developed as eco-friendly lubricant additives. These additives are tailored for use in environmentally acceptable lubricants (EALs) like polyalkylene glycols, vegetable oils, and synthetic esters.